6. Operating the infinite® 200
2008-07 Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4 111
In addition, the plate type (e.g. material of bottom of the microplate) also
influences the characteristics of the light beam. Therefore the theoretical border
displayed in the software might not correspond to the actual border when
measuring a real sample. It is therefore strongly recommended to optimize the
multiple reads per well parameters for every new application. Make sure that the
selected border ensures sufficient distance between light beam and wall of the
microplate well.
STOP
Caution
All absorbance and fluorescence intensity specifications given in this
document are only valid for single point measurements (one
measurement point per well). When using the multiple reads per well
option the specifications are not valid.
STOP
Caution
The software displays only a schematic view of the measurement
pattern. Therefore optimize the multiple reads per well parameters for
every new application. Make sure that the selected border
is sufficient to avoid an overlap between the light beam
and the well wall of the microplate.
STOP
Caution
A ‘border’ value that is too small may cause
wrong measurement results due to overlap between the light beam
and the well wall of the microplate.
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