tecan infinite 200 - Glow Luminescence

8. Quality Control
140 Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4 2008-07
8.3.5 Glow Luminescence
For the infinite
®
200 with the option ‘Luminescence the following tests may be
performed to prove the specifications:
• Sensitivity
Sensitivity
Perform the following measurement to determine the Sensitivity:
Measurement Parameters:
Parameter Setting
Reading Mode Luminescence
Integration Time 1000 ms
Settle Time 0
Plate Type GRE96fw
Part of the Plate A1 – D10
Plate Layout:
Pipette 200 µl of the ATP reagents into the appropriate wells according to the
plate layout:
1 2 3 4 5 6 7 8 9 10 11 12
A
Bx ATP Bx B B B B B B B
B
Bx ATP Bx B B B B B B B
C
Bx ATP Bx B B B B B B B
D
Bx ATP Bx B B B B B B B
E
F
G
H
ATP 2*10
-8
M ATP (final concentration in well)
B Blank (ATP reagent: Tris-EDTA=1:5)
Bx Blank (wells used for cross-talk calculation)
Material/Reagents:
BioThema ATP Kit (ATP-Kit SL 144-041, BioThema AB)
1 Greiner 96-well plate white
200 µl Pipette + tips