tecan infinite 200 - Time Resolved Fluorescence

8. Quality Control
136 Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4 2008-07
8.3.3 Time Resolved Fluorescence
For the infinite
®
200 with the option ‘Fluorescence Top’ the following tests may
be performed to prove the specifications:
• Sensitivity
• Precision/Repeatability
These test instructions are valid for the
infinite
®
200:
•
infinite
®
F200
• Spectrally enhanced version
Sensitivity
Perform the following measurement to determine the sensitivity:
Measurement Parameters:
Parameter Setting
Reading Mode Fluorescence Top
Exc Wavelength
infinite
®
F200: 340 nm (35)
infinite
®
M200: 340 nm
Em Wavelength
infinite
®
F200: 612 nm (10 nm)
infinite
®
M200: 617 nm
Number of flashes 25
Integration Time 400
Lag Time 100
Settle Time 0
Gain Optimal
Plate Type GRE96fw
Plate Layout:
Pipette 200 µl of 1 nm Europium solution or the blank solution (enhancement
solution) into the appropriate wells according to the plate layout:
<> 1 2 3 4 5 ...
A
B
C
D
E
F
G
H
1 nm Europium
Blank Blank Blank Blank