8. Quality Control
2008-07 Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4 137
Material/Reagents:
1 nm Europium (B119-100, HVD Live Sciences)
Enhancement Solution (=Blank) (1244-105, HVD Live Sciences)
1 Greiner 96-well plate white
200 µl Pipette + tips
Calculation of Detection Limit (Sensitivity):
See 8.3.1 Fluorescence Top: Sensitivity.
Precision
Perform the following measurement to determine the precision/reproducibility:
Measurement Parameters:
Parameter Setting
Reading Mode Fluorescence Top
Exc Wavelength
infinite
®
F200: 340 nm (35)
infinite
®
M200: 340 nm
Em Wavelength
infinite
®
F200: 612 nm (10 nm)
infinite
®
M200: 617 nm
Number of flashes 25
Integration Time 400
Lag Time 100
Time between Move and Flash 0
Gain Optimal
Plate Type GRE96fw
Part of the plate A1
Kinetic 20 Cycles
Interval Time Minimal
Plate Layout:
See 8.3.3 Time Resolved Fluorescence Precision.
Calculation of Precision:
See 8.3.1 Fluorescence Top: Precision.
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