tecan infinite 200 - Absorbance Accuracy

8. Quality Control
2008-07 Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4 141
Calculation of the Sensitivity (Detection Limit):
15
BATP
B
8
e1
1
*0002.0*
meanmean
Stdev*3*102
)well/fmol(imitDetectionL
−
−
−
⋅
=
2*10
-8
Concentration of ATP standard [M]
Stdev
B
Standard deviation of Blank
mean
ATP
Average of wells filled with ATP standard
mean
B
Average of Blank wells
0.0002 Conversion into mol/well
1/1e
-15
Conversion into fmol/well
The result of the formula determines the detection limit in fmol/well.
8.3.6 Absorbance Accuracy
Use MultiCheck Plate – For details please refer to the MultiCheck Instructions for
Use.