tecan infinite 200 - Luminescence Optics

5. Optical System
90 Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4 2008-07
5.7.1 Luminescence Optics
In luminescence measurement mode, the infinite
®
200 uses fixed microplate
position and a moveable luminescence measurement head (see Figure 5-13:
Opt
ical System Luminescence). The plate thickness is defined by selecting the
corresponding plate type in the software (see i-Control Instructions for Use).
Fiber
A glass fiber guides the light from the sample to the detection unit. The fiber is
designed to measure 96-well plates as well as 384-well plates.
Filter Wheel
A filter wheel with 6 filter positions in front of the PMT window is switched to the
required luminescence channel. The sensitivity of the detection system makes it
necessary to attenuate high luminescence light levels; therefore, the filter wheel
can also switch a neutral density filter across the selected fiber exit.
Filter Wheel Position Filter
Position 1 Lumi Green*
Position 2 Lumi Magenta*
Position 3 1 OD neutral density filter
Position 4 No attenuation
Position 5 Blue 1**
Position 6 Green 1**
* recommended for BRET ™2 and ChromaGlo™-Luciferase Assay
** recommended for BRET™ 1
See Figure 5-14 to Figure 5-1
7 for transmission spectra of luminescence filters.
0
10
20
30
40
50
60
70
80
90
100
400 450 500 550 600 650
Wavelength [nm]
Transmission [%
]
Figure 5-14: Transmission spectrum of filter ‘Lumi Magenta’