tecan infinite 200 - Fluorescence Polarization (Infinite F200 Only)

8. Quality Control
138 Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4 2008-07
8.3.4 Fluorescence Polarization (infinite
®
F200 only)
For the infinite
®
F200 with the option ‘Fluorescence Polarization’ the following tests
may be performed to prove the specifications:
• Precision/Repeatability
These test instructions are valid for the infinite
®
F200:
• Standard version
• Spectrally enhanced version
Precision
Perform the following measurement to determine the precision:
Measurement Parameters:
Parameter Setting
Reading Mode Fluorescence Polarization
Exc Wavelength 485 (20 nm)
Em Wavelength 535 (25 nm)
Number of flashes 25
Integration Time 40
Lag Time 0
Time between Move and Read 0
Gain Optimal
Plate Type GRE96fb
Reference from/to A1 – D1
Reference blank from/to A2 – D2
Reference value 20 mP
Measurement blank from/to same as reference blank