2. General Description
2008-07 Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4 25
2.3.4 Priming and Washing of the infinite
®
200
STOP
Caution
The injector carrier must be in the service position
for washing und priming.
Prime and Wash must not be performed
when the injector is in the instrument!
The initial filling step of the injector system (priming) as well as the cleaning step
of the injector system (washing) must take place outside of the instrument. For
these procedures the injector carrier is removed from instrument and put into the
service position of the injector box. For priming and washing steps of the injector
system, a default setting for injection speed and volume dispensed is provided. If
required the priming parameters can be adjusted in the injector control window of
the i-Control software.
The prime volume depends on the tubing length. Two types of injector tubing are
available: ‘long’: 105 cm, and ‘short’: 80 cm.
For the initial filling step of the injector system (priming) it is recommended to use
at least 2000 µl to remove all air bubbles from the injection system. The minimum
prime volume is therefore 2 ml. To save precious reagents, this initial filling step
can be performed with distilled water. To replace the water with the required
reagent, a second priming step is needed. For this second priming step, the
priming volume can be reduced to approx. 1500 µl.
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