5. Optical System
76 Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4 2008-07
5.4 Absorbance System ― infinite
®
M200
For absorbance measurements, a similar optical path is used as for fluorescence
excitation. For details of the light source (1) and the excitation monochromator
(2), please refer to 5.1.1 Light Source System Fluorescence Intensity. A fiber
bundl
e guides the light from the excitation monochromator to the absorbance
microplate (MTP) optics (3), which focuses the light into the wells. The
absorbance MTP measurement module (4) is located underneath the plate
carrier. These modules measure the light being transmitted through the sample.
Before measurement of the microplate, a reference measurement is performed
with the plate carrier moved out of the light beam.
Figure 5-6 Optical System Absorbance
infinite
®
M200
2
1
3
4
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