2. General Description
2008-07 Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4 43
2.4.2 i-Control Examples
Example 1: Dual Luciferase Assay™ (Promega Corp.)
For assay details please refer to www.promega.com.
Plate
Select an appropriate plate type. For luminescence measurements, white
microplates are recommended. For this example, a white 96-well plate was
selected.
Part of the plate
(Not shown); can be optionally selected if only part of the plate is to be
processed.
Well
Mandatory for measurements with ‘injection’
Injection (1)
Injector A injects 100 µl with speed 200 µl/sec., refill mode: standard
Wait (Timer)
2 s wait time
Luminescence (1)
Luminescence measurement with 10 s integration time, attenuation ‘none’
Injection (2)
Injector B injects 100 µl with speed 200 µl/sec., refill mode standard
Wait (Timer)
2 s wait time
Luminescence (2)
Luminescence measurement with 10 s integration time, attenuation ‘none’
Move Plate
Plate is moved out after finishing all wells
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