tecan infinite 200 - Fluorescence Bottom

8. Quality Control
2008-07 Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4 133
8.3.2 Fluorescence Bottom
For the infinite
®
200 with the option ‘Fluorescence Bottom’ the following tests
may be performed to prove the specifications:
• Sensitivity
• Uniformity
• Precision/Repeatability
These test instructions are valid for the
infinite
®
200:
•
infinite
®
F200
•
infinite
®
M200
• Standard version
• Spectrally enhanced version
Sensitivity
Perform the following measurement to determine the detection limit for
Fluorescein:
Measurement Parameters:
Parameter Setting
Reading Mode Fluorescence Bottom
Exc Wavelength
infinite
®
F200: 485 (20 nm)
infinite
®
M200: 485 nm
Em Wavelength
infinite
®
F200: 535 (25 nm)
infinite
®
M200: 535 nm
Number of flashes 25
Integration Time 40
Settle Time 0
Gain Optimal
Plate Type GRE96fb
Plate Layout:
<> 1 2 3 4 5 6 7 8 9 10 11 12
A
B
C
D
E
F
G
H
25 nm Fluorescein
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25 nm Fluorescein
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25 nm Fluorescein
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25 nm Fluorescein
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25 nm Fluorescein
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25 nm Fluorescein
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