tecan infinite 200 - Page 134

8. Quality Control
134 Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4 2008-07
Material/Reagents:
25 nm Fluorescein (in 0.01 M NaOH) (Fluorescein sodium salt, Sigma F6377)
0.01 M NaOH (=Blank) (NaOH pellets, Merck article no. 6495 or Sigma S8045)
1 Greiner 96-well plate µClear, black with transparent bottom
200 µl Pipette + tips
Calculation of Detection Limit (Sensitivity):
See 8.3.1 Fluorescence Top: Sensitivity.
Uniformity
Perform the following measurement to determine the Uniformity:
Measurement Parameters:
Parameter Setting
Reading Mode Fluorescence Bottom
Exc Wavelength
infinite
®
F200: 485 (20 nm)
infinite
®
M200: 485 nm
Em Wavelength
infinite
®
F200: 535 (25 nm)
infinite
®
M200: 535 nm
Number of flashes 25
Integration Time 40
Settle Time 0
Gain Optimal
Plate Type GRE96fb
Plate Layout:
<> 1 2 3 4 5 6 7 8 9 10 11 12
A
B
C
D
E
F
G
H
25 nm Fluorescein
Blank
25 nm Fluorescein
Blank
25 nm Fluorescein
Blank
25 nm Fluorescein
Blank
25 nm Fluorescein
Blank
25 nm Fluorescein
Blank
Filling volume: 200 µl
Material/Reagents:
25 nm Fluorescein (in 0.01 M NaOH) (Fluorescein sodium salt, Sigma F6377)
0.01 M NaOH (=Blank) (NaOH pellets, Merck article no. 6495 or Sigma S8045)
1 Greiner 96-well plate µClear, black with transparent bottom
200 µl Pipette + tips
Calculation of Uniformity:
See 8.3.1 Fluorescence Top: Uniformity