tecan infinite 200 - 7.4 Fluorescence Polarization (FP- infinite F200 only)

7. Instrument Features
2008-07 Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4 119
7.4 Fluorescence Polarization (FP- infinite
®
F200 only)
Note
The option ‘Fluorescence Polarization’ is only available for the
infinite
®
F200. This module cannot be installed on a infinite
®
M200.
Parameters Instrument type Characteristics
Wavelength Range standard Excitation: 300 – 600 nm
spectrally-enhanced Excitation: 300 – 850 nm
standard Emission: 330 – 600 nm
spectrally-enhanced Emission: 330 – 850 nm
Standard Filter both Configuration of Default filter
slide:
Exc 485 (20) nm
Position on Exc filter slide:
Exc1: 485 (20) - parallel
Exc2: 485 (20) - perpendicular
Em 535 (25) nm
Position on Em filter slide:
Em1: 535 (25) - parallel
Em2: 535 (25) - parallel
Gain Setting Values Measurement Range
Manual 1 - 255 0 - 60,000 RFU
Optimal automatic 0 - 60,000 RFU
Calculated from Well automatic 0 - 60,000 RFU
FP Parameters Characteristics
Integration Time 20 - 2000 µs
Lag Time 0 - 2000 µs
FP Precision (F200 only) < 5 mP @ 1nM Fluorescein