5. Optical System
2008-07 Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4 79
Note
The cuvette port is an option of the infinite
®
M200 only. This option is not
available for infinite
®
F200. With the infinite
®
F200, cuvettes may be
measured using a Tecan Cuvette Adapter placed on the plate transport.
5.5.1 Absorbance Optics Cuvette
A fiber bundle guides the light from the excitation monochromator system to the
absorbance cuvette optics.
This optics consists of a pair of lenses focusing the light beam into the cuvette.
At the focal point, the spot diameter of the absorbance light beam is 1.9 mm.
5.5.2 Absorbance Detection Cuvette
A silicon photodiode is used for the measurement of the transmitted light. It is
sensitive to a wide range of wavelengths. The photodiode is well suited for the
light levels being encountered with absorbance measurements below 3 O.D.
Measurement values above 3 OD are marked as ‘OVER’ in the result sheet.