tecan infinite 200 - 8.3 Specifications - Test Instructions

8. Quality Control
2008-07 Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4 129
8.3 Specifications - Test Instructions
8.3.1 Fluorescence Top
For the infinite
®
200 with the option ‘Fluorescence Top’, the following tests can
be performed to prove the specifications:
• Sensitivity
• Uniformity
• Precision
These test instructions are valid for the
infinite
®
200:
•
infinite
®
F200
•
infinite
®
M200
• Standard version
• Spectrally enhanced version
Sensitivity
Perform the following measurement to determine the detection limit for
Fluorescein:
Measurement Parameters:
Parameter Setting
Reading Mode Fluorescence Top
Exc Wavelength
infinite
®
F200: 485 (20 nm)
infinite
®
M200: 485 nm
Em Wavelength
infinite
®
F200: 535 (25 nm)
infinite
®
M200: 535 nm
Number of flashes 25
Integration Time 40
Settle Time 0
Gain Optimal
Plate Type GRE96fb