tecan infinite 200 - FI Ratio Mode

6. Operating the infinite® 200
98 Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4 2008-07
6.3.2 FI Ratio Mode
Ratio Mode
Up to 4 labels may be measured well-wise. This measurement mode is called
‘ratio mode’. Be aware that no ‘ratio’ calculation is performed after this
measurement. The Excel
®
result sheet shows the raw data. Further calculations
have to be performed by the user.
Filter Switch Time (infinite
®
F200)/Wavelength Switch Time (infinite
®
M200)
The infinite
®
F200 can switch between two filters within 250 ms in case that the
selected labels are measured with the same gain. Otherwise, the switching time
is 400 ms. In this case the high voltage level at the PMT needs to be changed.
The high voltage applied to the PMT needs some time to stabilize.
The
infinite
®
M200 can switch between two wavelengths within 150 ms in case
that the selected labels are measured with the same gain and no order sorting
(OS) switching point is involved (see Table 6.3-1:for switching points). Otherwise,
the swit
ching time is 400 ms. In this case the high voltage level at the PMT needs
to be changed. The high voltage applied to the PMT needs some time to stabilize.
The OS filter wheel needs to be moved.
Table 6.3-1: OSF (Order Sorting Filter) Switching Points (infinite
®
M200)
Excitation Wavelength Emission Wavelength
OSF Switching Point 1
316 nm 401 nm
OSF Switching Point 2
386 nm 621 nm
OSF Switching Point 3
561 nm -
Example:
Fura-2: This application involves a filter/wavelength switch between 340 and
380 nm on the excitation side. The emission is measured at about 510 nm. The
excitation filter/wavelength switch does not include an OS switch, therefore the
switch is possible within 150 ms on an infinite
®
M200 and 250 ms on a
infinite
®
F200.