Agena BIOSCIENCE MassARRAY Nanodispenser RS1000 User Manual

Appendix D Troubleshooting152
USG-CUS-059 Rev05MassARRAY® Nanodispenser RS1000 v2.1 User Guide
For Research Use Only. Not for use in diagnostic procedures.
Dispensed volume too
low.
During a run: volumes are shown in
the Volume tab of the Transfer
screen.
After a run: A warning message
appears if any dispenses were out
of range.
Increase dispense speed. To set the dispensing
speed:, page 37
At the desalting step, use a 0.001%
Tween and HPLC-grade water
solution in place of the regular water
addition.
Desalting the Analyte,
page 21
For UltraSEEK panels only: re-
dispense analyte from the reaction
plate onto the SpectroCHIP Array,
i.e., double dispense the analyte
onto the same pads. This increases
the number of analyte molecules,
thereby increasing signal intensity.
Use the final dispensed volume
from the first dispensing run as the
initial volume for the second
dispensing run.
Dispensing to
SpectroCHIP Arrays,
page 19
and
Accessing dispense
volume data from
previous runs, page 56
Dispensed volume too
variable.
Transfer tab will show a CV(%) that is
too high.
Increase aspirate time and/or
dispense time.
Recommended
Dispense Settings,
page 31
Selecting aspirate/
dispense parameters,
page 37
At the desalting step, use a 0.001%
Tween and HPLC-grade water
solution in place of the regular water
addition.
Incorrect number of
fiducials (i.e., incorrect
SpectroCHIP Array
alignment)
Warning message appears at start
of dispensing run.
Ensure that the SpectroCHIP Array
is properly seated on the deck.
If that does not solve the problem,
retry with a new SpectroCHIP Array.
Loading the
SpectroCHIP Arrays,
page 45
Removing the
SpectroCHIP Arrays,
page 54
Unable to read plate
barcode.
Warning message appears at start
of dispensing run.
Ensure that the recommended plate
type is being used.
Attempt to restart dispensing run.
If that does not solve the problem,
retry with a new plate with a new
barcode.
Table 1.8
Tap the run button on the
Transfer screen.
Setting up the
dispensing run, page 30
Unable to read
SpectroCHIP Array
barcode.
Warning message appears at start
of dispensing run.
Attempt to restart dispensing run.
If that does not solve the problem,
retry with a new SpectroCHIP Array.
Tap the run button on the
Transfer screen.
Setting up the
dispensing run, page 30
Issue Notification Location Recommended Action See

Agena BIOSCIENCE MassARRAY Nanodispenser RS1000 Specifications

General

Brand	Agena BIOSCIENCE
Model	MassARRAY Nanodispenser RS1000
Category	Measuring Instruments
Language	English