Agena BIOSCIENCE MassARRAY Nanodispenser RS1000 - Chapter 2 Dispensing to Spectrochip Arrays; Dispensing Overview

USG-CUS-059 Rev05MassARRAY® Nanodispenser RS1000 v2.1 User Guide
For Research Use Only. Not for use in diagnostic procedures.
Chapter 2
Dispensing to SpectroCHIP Arrays
Dispensing Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Desalting the Analyte . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Instrument start up and shut down . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Pre-run checks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Setting up the dispensing run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Microtiter plate checks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Setting up the instrument deck . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Starting the dispensing run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Dispensing run activities . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Clearing the instrument deck . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
Accessing dispense volume data from previous runs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
2.1 Dispensing Overview
Table 2.1 Dispensing Workflow
Step See Page
1. Desalt the analyte. 21
2. Turn on the Nanodispenser RS1000 and log on. 22
3. Pre-run checks:
• Confirm that the daily maintenance procedures have been completed. 91
• Check the supply and waste tank status. 28
• Perform pin priming (optional). 29
4. Setup the dispensing run in the software:
• Load a method. 32
• Select setup parameters. 34
• Select cleaning parameters. 37
• Select aspirate/dispense parameters. 37
5. Microtiter plate checks
• Check resin and liquid levels. 39
• Determine the aspirate offset 40
6. Set up the instrument deck.
• Fill the calibrant reservoir. 43
• Load the microtiter plates. 44
• Load the SpectroCHIP Arrays. 45
7. Start the dispensing run. 46
8. Clear the instrument deck.
• Remove the SpectroCHIP Arrays. 54
• Remove the microtiter plates. 55