Chapter 2 Dispensing to SpectroCHIP Arrays20
USG-CUS-059 Rev05MassARRAY® Nanodispenser RS1000 v2.1 User Guide
For Research Use Only. Not for use in diagnostic procedures.
This chapter explains how to desalt the analyte with resin, and then dispense analyte and
calibrant to SpectroCHIP Arrays. Table 2.1 lists the workflow steps. The Nanodispenser
RS1000 loads analytes from the microtiter plates located on the instrument processing
deck (two plates maximum). Calibrant is loaded from a reservoir on the processing deck.
Analytes refers to iPLEX
®
, MassCLEAVE
TM
, or UltraSEEK
TM
biochemistry products.
Calibrant is a mixture of oligonucleotides of known masses that is used to calibrate the
MassARRAY Analyzer.
NOTENOTE
The pins should be left submerged in the wash solution whenever the instrument is not in use. See
page 90 for instructions on soaking the pins.
Example dispensing
cycle
Figure 2.1 shows an example dispensing cycle and deck setup with a single microtiter
plate and SpectroCHIP Array on the deck.
The cycle (step 1 through step 6) is repeated until all of the wells in the microtiter plate are
dispensed to the SpectroCHIP Array. For example, on a 24-pin instrument which
processes a maximum of 24 wells at a time, the cycle will repeat 16 times to process all of
the wells in a 384-well microtiter plate.
NOTENOTE
The example dispensing cycle shown in Figure 2.1 is intended to illustrate the basic movements
that the instrument performs during processing. Actual dispensing scripts may differ.
• Empty the calibrant reservoir. 55
9. Log off and turn off the Nanodispenser RS1000. 23
Step See Page
Figure 2.1 Example Dispense Cycle and Deck Components
Dry station
Microtiter plate position
SpectroCHIP Arrays
Ultrasonic wash
Rinse station
Dispense cycle steps:
1. Rinse pins
2. Dry pins
3. Wash pins
4. Dry pins
5. Load fluid from microtiter
plate
6. Dispense fluid to
SpectroCHIP Array
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