USG-CUS-059 Rev05MassARRAY® Nanodispenser RS1000 v2.1 User Guide
For Research Use Only. Not for use in diagnostic procedures.
Contents
Chapter 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . 3
Overview . . . . . . . . . . . . . . . . . . . . . . . 3
Instrument Description . . . . . . . . . . . . . . . . . . . 4
Required and Recommended Equipment and Consumables . . . . . . 13
Safety. . . . . . . . . . . . . . . . . . . . . . . . . 14
Contacting Agena Bioscience . . . . . . . . . . . . . . . . 17
Chapter 2 Dispensing to SpectroCHIP Arrays . . . . . . . . . . . . . . . 19
Dispensing Overview . . . . . . . . . . . . . . . . . . . 19
Desalting the Analyte . . . . . . . . . . . . . . . . . . . 21
Instrument start up and shut down . . . . . . . . . . . . . . . 22
Pre-run checks. . . . . . . . . . . . . . . . . . . . . . 28
Setting up the dispensing run. . . . . . . . . . . . . . . . . 30
Microtiter plate checks . . . . . . . . . . . . . . . . . . . 39
Setting up the instrument deck . . . . . . . . . . . . . . . . 43
Starting the dispensing run . . . . . . . . . . . . . . . . . 46
Dispensing run activities . . . . . . . . . . . . . . . . . . 49
Clearing the instrument deck . . . . . . . . . . . . . . . . . 54
Accessing dispense volume data from previous runs . . . . . . . . . 56
Chapter 3 Methods and Mappings . . . . . . . . . . . . . . . . . . . . 57
About method files . . . . . . . . . . . . . . . . . . . . 57
About mapping files . . . . . . . . . . . . . . . . . . . . 58
Creating a custom method file . . . . . . . . . . . . . . . . 63
Deleting a method file . . . . . . . . . . . . . . . . . . . 73
Creating a custom mapping file . . . . . . . . . . . . . . . . 74
Deleting a mapping file . . . . . . . . . . . . . . . . . . . 86
Chapter 4 Maintenance Procedures . . . . . . . . . . . . . . . . . . . 89
Tank valve positions . . . . . . . . . . . . . . . . . . . . 89
Maintenance overview . . . . . . . . . . . . . . . . . . . 90
Pin soak . . . . . . . . . . . . . . . . . . . . . . . . 90
Daily maintenance . . . . . . . . . . . . . . . . . . . . 91
Monthly maintenance . . . . . . . . . . . . . . . . . . . 104
Quarterly maintenance . . . . . . . . . . . . . . . . . . . 111
Repairs . . . . . . . . . . . . . . . . . . . . . . . . 116
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