EasyManua.ls Logo

Agilent Technologies G1313A User Manual

Agilent Technologies G1313A
292 pages
To Next Page IconTo Next Page
To Next Page IconTo Next Page
To Previous Page IconTo Previous Page
To Previous Page IconTo Previous Page
Page #42 background imageLoading...
Page #42 background image
42
Optimizing Performance
Optimization for Lowest Carry-over
Injector Program with Needle Wash
The injector program includes the command NEEDLE WASH. When this
command is included in the injector program, the needle is lowered once into
the specified wash vial before injection.
For example:
1 DRAW 5 µl
2 NEEDLE WASH vial 7
3 INJECT
Line 1 draws 5 µl from the current sample vial. Line 2 moves the needle to vial
7. Line 3 injects the sample (valve switches to main pass).
Using an Injector Program
The process is based on a program that switches the bypass grove of the
injection valve into the flow line for cleaning. This switching event is
performed at the end of the equilibration time to ensure that the bypass grove
is filled with the start concentration of the mobile phase. Otherwise the
separation could be influenced, especially if microbore columns are used.
For example:
Outside wash of needle before injection: 14 sec. using flush port
Injector program:
Draw x.x (y) µl from sample
Needle wash as method
Inject
Wait (equilibration time - see text above)
Valve bypass
Wait 0.2 min
Valve mainpass
Valve bypass
Valve mainpass

Table of Contents

Question and Answer IconNeed help?

Do you have a question about the Agilent Technologies G1313A and is the answer not in the manual?

Agilent Technologies G1313A Specifications

General IconGeneral
BrandAgilent Technologies
ModelG1313A
CategoryLaboratory Equipment
LanguageEnglish

Summary

Installing the Autosampler

Site Requirements

Details on the environmental and physical requirements for installing the autosampler, including power and bench space.

Unpacking the Autosampler

Guidance on inspecting the packaging and contents upon receiving the autosampler.

Optimizing the Stack Configuration

Recommendations for arranging autosampler modules in a stack for optimal system performance and flow path.

Installing the Autosampler

Step-by-step instructions for the physical installation of the autosampler unit.

Installing the Thermostatted Autosampler

Specific installation steps for the thermostatted version of the autosampler module.

Flow Connections

Instructions for connecting the capillary tubing for fluid flow within the autosampler system.

Sample Trays

Information on installing and using different types of sample trays with the autosampler.

Choice of Vials and Caps

Guidance on selecting compatible vials and caps for reliable autosampler operation.

Transporting the Autosampler

Procedures and precautions for safely moving the autosampler unit.

Optimizing Performance

Optimization for Lowest Carry-over

Techniques and settings to minimize carry-over for accurate quantitative analysis.

Fast Injection Cycle and Low Delay Volume

Strategies to shorten injection cycle times and reduce system delay volume for high throughput.

Precise Injection Volume

Methods and parameters for achieving accurate and precise sample injection volumes.

Choice of Rotor Seal

Information on selecting the appropriate rotor seal material for different mobile phases and conditions.

Troubleshooting and Test Functions

Status Indicators

Explanation of the instrument status indicators and their meanings.

Error Messages

Detailed descriptions of autosampler error messages, probable causes, and suggested actions.

Maintenance Functions

Overview of maintenance functions for positioning internal components for service.

Tray Alignment

Procedure for aligning the gripper arm after repairs or firmware updates.

Step Commands

Manual control of sampling sequence steps for troubleshooting and verification.

Troubleshooting Guide for the Sample Transport Assembly

Guide to diagnosing and repairing common autosampler transport assembly problems.

Repairing the Autosampler

Simple Repairs

Procedures for common repairs that can be done with the autosampler in place.

Exchanging Internal Parts

Procedures for exchanging internal components, requiring removal from the stack.

Updating the Firmware

Instructions on how to update the instrument firmware.

Cleaning the Autosampler

Guidelines for cleaning the exterior covers of the autosampler.

Using the ESD Strap

Procedure for correctly using an ESD strap to prevent damage to electronic components.

Overview of Main Repair Procedures

Summary of major repair procedures with references to detailed instructions.

Parts and Materials

Main Assemblies

Diagram and list of major assemblies within the autosampler.

Sampling Unit Assembly

Diagram and part numbers for the autosampler sampling unit assembly.

Analytical-Head Assembly

Details and part numbers for different analytical head assemblies.

Injection-Valve Assembly

Assembly details and part numbers for the autosampler injection valve.

Sheet Metal Kit

List of parts included in the sheet metal kit.

Cover Parts

Part numbers and descriptions for various cover components.

Foam Parts

Part numbers and descriptions for foam components.

Power and Status Light Pipes

Part numbers for power and status light pipe components.

Leak System Parts

Part numbers and descriptions for components of the leak detection system.

Vial Trays

Part numbers and descriptions for autosampler vial trays and tray bases.

Autosampler Accessory Kit G1313-68705

Contents and part numbers for the standard autosampler accessory kit.

Thermostatted Autosampler Accessory Kit G1329-68705

Contents and part numbers for the thermostatted autosampler accessory kit.

Micro Thermostatted Autosampler Accessory Kit G1329-68715

Contents and part numbers for the micro thermostatted autosampler accessory kit.

Preparative ALS Accessory Kit G2260-68705

Contents and part numbers for the preparative autosampler accessory kit.

Maintenance Kit G1313-68709

Contents and part numbers for the maintenance kit.

Multi-Draw Kit G1313-68711

Contents and part numbers for the multi-draw kit.

900 µl Injection Upgrade Kit G1363A for G1313A / 29A

Contents and part numbers for the 900 µl injection upgrade kit.

External Tray G1313-60004

Contents and part numbers for the external tray.

Cable Overview

Listing and description of various cables used with the Agilent 1100 Series modules.

Analog Cables

Details and pin assignments for analog signal output cables.

Remote Cables

Details and pin assignments for remote control cables.

Auxiliary Cable

Details and pin assignments for the auxiliary cable.

CAN Cable

Specifications for CAN bus cables connecting Agilent 1100 modules.

External Contact Cable

Details and pin assignments for external contact interface cables.

RS-232 Cable Kit

Contents and connection details for the RS-232 cable kit.

LAN Cables

Recommendations for LAN cables used for network connections.

Introduction to the Autosampler

Introduction to the Autosampler

General overview of the Agilent 1100 Series autosamplers, their models, and system integration.

Sampling Sequence

Detailed description of the automated sampling sequence steps.

Injection Sequence

Explanation of the mainpass and bypass positions during the injection sequence.

Sampling Unit

Description of the sampling unit and its main assemblies.

Theory of Operation

Autosampler Control and Electronics

Overview of the electronics and processor controlling autosampler functions.

Position and Movement Sensors

Details on sensors used for position sensing on flex boards.

Autosampler Main Board (ASM)

Description of the common electronics, ASIC, and leak converter on the main board.

Firmware Description

Explanation of the instrument's firmware structure and properties.

Optional Interface Boards

Information on optional interface boards like BCD and LAN boards.

Interfaces

Overview of the different interfaces available on Agilent 1100 Series modules.

RS-232C

Details on the RS-232C connector, its pins, and cable kit.

Setting the 8-bit Configuration Switch

Instructions for configuring the instrument using the 8-bit configuration switch.

The Main Power Supply Assembly

Description and specifications of the autosampler's main power supply unit.

Introduction to the Control Module

Screens available from the Analysis screen

Description of the analysis screen and its related functions like setup and method parameters.

Screens available from the System screen

Overview of the system screen, control, and configuration options.

Screens available from the Records screen

Details on accessing records, logs, and setting maintenance parameters.

Diagnostics and Tests

Information on running diagnostic tests and accessing schematics.

Specifications

Performance Specifications

Detailed performance specifications for Agilent 1100 Series Autosamplers.

Legal, Safety and Warranty Information

Warranty Statement

Terms and conditions of the Agilent Technologies warranty.

Safety Information

General safety precautions for operation, service, and repair of the instrument.

Lithium Batteries Information

Information and warnings regarding the handling and disposal of lithium batteries.

Radio Interference

Guidelines regarding the use of cables and compliance with EMC regulations.

Sound Emission

Manufacturer's declaration regarding sound pressure emission levels.

Solvent Information

Recommendations and warnings for the use of various solvents.

Agilent Technologies on Internet

Information on accessing the Agilent Technologies website for product and service information.

Related product manuals