Chapter 6 Performing the Pure Dye Calibration
Evaluating Pure Dye Calibration Spectra
82 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Notes
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Table 2 Spectra for pure dyes supported by the 7500 system
Instrument Pure Dye Filter Peak (nm)
7500 System FAM A ~520 nm
JOE B ~550 nm
NED C ~550 nm
ROX D ~610 nm
SYBR
Green
A ~520 nm