Chapter 6 Performing the Pure Dye Calibration
Evaluating Pure Dye Calibration Spectra
84 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Notes
Table 3 Spectra for pure dyes supported by the 7500 Fast system
Instrument Pure Dye Filter Peak (nm)
7500 Fast System FAM A ~520 nm
JOE B ~550 nm
NED C ~550 nm
ROX D ~610 nm
SYBR
Green
A ~520 nm