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7300
Applied Biosystems 7300 User Manual
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Chapter 6
Performing the Pure Dye Calibration
Evaluating Pure Dye C
alibration Spectra
86
Applied Biosystems 7300/7
500/7500 Fast Real-Ti
me PCR System Installation an
d Maintenance Gu
ide
Notes
111
113
Table of Contents
Default Chapter
5
7300/7500/7500 Fast System
5
Installation and Maintenance Workflow
5
Table of Contents
7
How to Use this Guide
11
Preface
11
How to Obtain more Information
12
How to Obtain Support
13
Safety and EMC Compliance Information
16
Safety Conventions Used in this Document
16
Symbols on Instruments
17
Safety Labels on Instruments
18
General Instrument Safety
20
Chemical Safety
21
Chemical Waste Safety
22
Electrical Safety
23
Biological Hazard Safety
24
Physical Hazard Safety
24
Safety and Electromagnetic Compatibility (EMC) Standards
25
Workstation Safety
25
Before Starting the Installation
27
Chapter 1 Before Starting the Installation
28
Selecting the Installation Method
28
Setting up the Computer
28
Planning the Installation
31
Unpacking and Setting up the Instrument
33
Before You Begin
34
Chapter 2 Unpacking and Setting up the Instrument
34
Selecting Additional Hardware and Software
35
Uncrating and Placing the 7300/7500/7500 Fast System
38
Checking the Shipped Materials
40
Setting up the 7300/7500/7500 Fast Instrument
46
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
47
Installing the Software and Powering on the Instrument
49
Chapter 3 Installing the Software and Powering on the Instrument
50
Overview
50
Installing the SDS Software for the 7300/7500/7500 Fast System
51
Powering on the Instrument
54
Chapter 4 Performing the Instrument Function Test
56
Troubleshooting
58
Performing the Regions of Interest (ROI) Calibration
61
Overview
62
Preparing the ROI Calibration Plate
64
Creating a Plate Document for the ROI Calibration
67
Performing the ROI Calibration
70
Troubleshooting
74
Performing the Background Calibration and Optical Calibration
75
Background Calibration and Optical Calibration
76
Overview
76
Preparing the Background Calibration Plate
78
Creating a Plate Document for the Background Calibration
80
Performing the Background Calibration
81
Analyzing the Background Calibration Data
82
Analyzing the Background Calibration Data
83
Performing the Optical Calibration for the 7500/7500 Fast System
85
Troubleshooting
90
Performing the Pure Dye Calibration
93
Chapter 6 Performing the Pure Dye Calibration
94
Overview
94
Preparing for the Calibration
97
Creating a Plate Document for the Pure Dye Calibration
98
Preparing Plates and Performing the Pure Dye Calibration
99
Analyzing the Pure Dye Calibration Data
103
Evaluating Pure Dye Calibration Spectra
106
Verifying the Instrument Performance
113
Chapter 7 Verifying the Instrument Performance
114
Overview
114
Preparing the Taqman Rnase P Verification Plate
116
Creating a Plate Document for the Rnase P Verification Run
118
Chapter 5 Performing the Verification Run
119
Analyzing the Taqman Rnase P Plate Data
120
Troubleshooting
125
Powering off the 7300/7500/7500 Fast System
127
Performing a Short-Term Shutdown
128
Performing a Long-Term Shutdown
130
Appendix A Maintaining the Instrument
131
Maintaining the Instrument
131
Recommended Maintenance Schedule
131
Recommended Maintenance Schedule
132
Archiving and Backing up SDS Files
133
Decontaminating the Sample Block
134
Cleaning up and Defragmenting the Hard Drive
140
Defragmenting the Hard Drive
140
Moving the 7300/7500/7500 Fast System
141
Monitoring Lamp Status
143
Replacing the Halogen Lamp
144
Replacing the Instrument Fuses
148
Updating the Operating System Software and Service Packs
150
Appendix B Creating a Background Plate
151
Creating a Background Plate
151
Overview
151
Appendix C Creating a Custom Pure Dye Plate
153
Overview
153
Determining Optimum Dye Concentration
154
Creating a Custom Pure Dye Plate
156
Adding the Custom Dye to the Software
157
Setting up the Computer
159
Appendix D Setting up the Computer
160
Unpacking the Computer and Setting Voltage
160
Connecting Components and Powering up
162
Setting the Display Settings and Power Options
163
Connecting to the Network and Downloading Adobe Acrobat
166
Index
167
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Applied Biosystems 7300 Specifications
General
Brand
Applied Biosystems
Model
7300
Category
Laboratory Equipment
Language
English
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