Applied Biosystems 7300 User Manual

Chapter 1 Before Starting the Installation
Planning the Installation
6 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Notes
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02:00:00
7300/7500
Perform the pure dye calibration (Chapter 6):
a. Prepare the
pure dye plates.
b. Create a plate document.
c. Perform the
pure dye calibration.
d. Analyze the pure dye calibration data.
e. Evaluate pure dye calibration spectra.
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01:20:00
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01:00:00
Verify instrument performance (Chapter 7):
a. Prepare the TaqMan
®
RNase P verification plate.
b. Create a plate document.
c. Perform the
verification run.
d. Analyze the TaqMan
®
RNase P plate data.
7500 Fast
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00:40:00
7500/7500 Fast7300
Note: You must be present at the instrument for approximately 5 minutes of the total run time.
7500 Fast
Recommended Workflow (continued)
See Note below
Perform the optical calibration (7500/7500 Fast systems only,
Chapter 5):
a. Create a plate document.
b. Perform the optical calibration.
c. Analyze the optical calibration data.
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00:10:00

Perform the background calibration (Chapter 5):
a. Prepare the
background calibration plate.
b. Create a plate document.
c. Perform the background calibration.
d. Analyze the background data.
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00:30:00
Time Required Task

Applied Biosystems 7300 Specifications

General

Brand	Applied Biosystems
Model	7300
Category	Laboratory Equipment
Language	English