Applied Biosystems 7300 User Manual

Chapter 4 Performing the Regions of Interest (ROI) Calibration
Performing the ROI Calibration
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide 47
Notes
4
3. Press the tray door to move the tray into the
instrument.
4. Place the calibration plate inside its packaging
sleeve.
5. 7300 systems: Return the packaged plate to the
spectral calibration kit in the freezer.
7500/7500 Fast systems:
– If you will perform background and optical
calibrations (described in the next chapter)
within the next 8 hours, keep the ROI
calibration plate at room temperature in the
packaging. The optical calibration you
perform after background calibration uses the
ROI calibration plate.
– If you be perform background and optical
calibrations on another day, return the
packaged plate to the spectral calibration kit in
the freezer.
Note: Do not discard the ROI calibration plate.
If you store the plate in its packaging sleeve at
–20 to –25 ° C, you can use the plate up to three
times after you open it.
Continue with “Performing the Background
Calibration and Optical Calibration” on page 49.
IMPORTANT! After you perform an ROI calibration,
you must also perform a background calibration (see
page 49), an optical calibration (7500 systems only,
see page 59), and pure dye calibrations (see page 67),
and instrument verification (see page 87).
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Applied Biosystems 7300 Specifications

General

Brand	Applied Biosystems
Model	7300
Category	Laboratory Equipment
Language	English