Applied Biosystems 7300 User Manual

Chapter 5 Performing the Background Calibration and Optical Calibration
Preparing the Background Calibration Plate
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide 53
Notes
5
7. Verify that the liquid in each well of the
background plate is at the bottom of the well. If
not, centrifuge the plate again at a higher rpm
and for a longer period of time.
IMPORTANT! Do not allow the bottom of the
background plate to become dirty. Fluids and
other contaminants that adhere to the bottom of
the plate can contaminate the sample block and
cause an abnormally high background signal.
Continue with “Creating a Plate Document for the
Background Calibration” on page 54.
Correct Incorrect
Liquid is at
bottom of well.
• Not centrifuged with enough force,
or
• Not centrifuged for enough time

Applied Biosystems 7300 Specifications

General

Brand	Applied Biosystems
Model	7300
Category	Laboratory Equipment
Language	English