Applied Biosystems 7300 User Manual

Chapter 5 Performing the Background Calibration and Optical Calibration
Troubleshooting
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide 65
Notes
5
4. Locate the contaminated well position(s) by selecting
successively fewer wells in the plate document.
The items that follow show an example of how to determine
the location of a contaminated well.
a. In the Spectra tab, select columns 1-6. The raw data from
the selected wells does not include the irregular peak.
Therefore, the contaminated well must be in columns 7-12.
b. Select columns 7-9. The raw data from the selected wells
includes the irregular peak. The contaminated well must be
in columns 7-9.
c. Select wells in row E and below in columns 7-9. The raw
data from the selected wells does not include the irregular
peak. The contaminated well must be in the first four wells
of columns 7-9.
d. Finally, by selecting each of the wells from the first four
wells of columns 7-9, you can determine the location of the
contaminated well (B9).
5. Repeat step 4 until you identify the location of each
contaminated well.
Troubleshooting – Background Calibration
Contaminated well
is not displayed
Contaminated
well
Contaminated
well is not
displayed
Contaminated
well
Contaminated
well

Applied Biosystems 7300 Specifications

General

Brand	Applied Biosystems
Model	7300
Category	Laboratory Equipment
Language	English