Illustration of fractions and fraction marking using peak fractionation with
Fraction collector F9-R
The following illustration shows the fractions collected, and the numerical marking of
fractions, when peak fractionation is used with Fraction collector F9-R.
Volume (ml)
Absorbance
Volume
2 3 4 6 7 9 10 11
Fractionation marks shown in chromatogram
When peak fractionation is used, Fraction collector F9-R collects the delay volumes in
the fractions preceeding each peak. In the chromatogram above, delay volumes are
collected in tubes corresponding to fraction 1, 5 and 8. The numerical fraction marks for
the delay volume fractions are not indicated on the chromatogram.
The built-in fraction collector does not save the delay volume, instead the delay
volume is disposed of in the waste funnel.
Note:
The fractions collected by Fraction collector F9-R, are indicated by the prefix
2: (i.e. fractions, 2:2, 2:3 etc.).
Note:
Fractionation modes for built-in
fraction collector
There are three fractionation modes for the built-in Fraction collector, Automatic, Accu-
mulator and DropSync. Spillage between collection vessels during a run is avoided with
all three fractionation modes.
•
Automatic: The fraction collector uses the DropSync mode for flow rates up to 2
ml/min and automatically switches to Accumulator mode for higher flow rates.
•
Accumulator: Liquid is collected during movement between tubes or wells. The liquid
is then dispensed in the next well or tube. Fractionation with accumulator can be
used at all flow rates.
•
DropSync: When using DropSync , the sensors in the Dispenser head detect when
a drop is released from the nozzle. The dispenser head moves to the next well or
tube just after a drop is released. Fractionation with DropSync can be used at flow
rates up to 2 ml/min. Solutions with low surface tension may require a lower flow.
222 ÄKTA avant User Manual 29035184 AE
5 Operation
5.8 Fractionation
5.8.3 Fractionation overview
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