ActionStep
In the System Settings dialog box:7
•
Select Fraction collection:Fractionation settings frac 2.
•
In the Drop sync field, click On.
•
Click OK.
Run and evaluate the test
Follow the instructions described in Section 6.1 General performance test actions, on
page 240 to start and run the performance test. See the following topic for instructions
on how to evaluate the fraction collector test.
Evaluate the result
Check that the correct volumes have been collected in the tubes. The tubes should
contain the following:
•
Tube 1: The delay volume
•
Tube 2, 3 and 4: 2 ml
•
Tube 5 and 6: 1 ml
Also, check that the fractionation marks in the chromatogram correspond to the filled
tubes and that spillage is kept to a minimum.
For further information on delay volumes and fractionation marks, see Delay volume,
on page 220.
ÄKTA avant User Manual 29035184 AE 251
6 Performance tests
6.4 Fraction collector F9-R test
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