Faulty Step Response Result
ActionCause
Air in pumps: Make sure to prime the buffer inlets and to
purge the System pumps and the Sample pump before
method start. See Section 5.4.1 Prime buffer inlets and
purge system pumps, on page166 and Section 5.4.2 Prime
sample inlets and purge Sample Pump, on page 173.
If all values are faulty -
air in the pump or a
faulty pump
Faulty pump. See Section 8.6 Troubleshooting: Pumps, on
page 397.
Replace piston seals. See ÄKTA avant Operating
Instructions, Maintenance chapter.
Faulty values at 5% -
damaged pump piston
seal in System pump B
Replace piston seals. See ÄKTA avant Operating
Instructions, Maintenance chapter.
Faulty values at 95% -
damaged pump piston
seal in System pump A
Faulty UV Absorbance Test
ActionCause
Make sure that the acetone solution is 1% and that no
solution has evaporated.
Incorrectly prepared
acetone solution
Faulty Pulsation Test
ActionCause
Make sure to prime and purge the system pumps before
starting the test, see Section 5.4.1 Prime buffer inlets and
purge system pumps, on page 166.
Air trapped in the
pumps
Faulty Conductivity Test Result
ActionCause
Make sure that the NaCl solution is 1.00 M.Incorrectly prepared
NaCl solution
258 ÄKTA avant User Manual 29035184 AE
6 Performance tests
6.6 System test
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