Prepare for calibration
Follow the instructions to prepare for the calibration of the UV monitor U9-L.
ActionStep
Ensure that the flow restrictor is inline in the flow path after the UV flow cell.1
Mount the union Luer female/1/16” male, included in the test kit, in the upper
inlet of the UV flow cell.
2
Open the UV-900 cell calibration Excel-file.3
The solution bottles are labelled with the concentration value and the refer-
ence absorbance value for each solution. Enter the concentrations of the
solutions in ascending order into the column UV Test kit Concentration
(mg/l). Enter the corresponding absorbance values into the column UV Test
kit Absorbance (AU/cm).
4
Perform the calibration
Follow the instructions to calibrate the second UV Monitor U9-L.
ActionStep
Open the System Control module. On the Manual menu, click Execute
Manual Instructions.
1
Result: The Manual instructions dialog box opens.
In the Manual Instructions dialog box:
•
Select Pumps:System flow and set the Flow rate to 0.0 ml/min.
•
Click Execute.
Result: The absorbance can now be monitored.
Fill one of the supplied syringes with 1.5-2 ml of the first solution (0 mg/l).
Ensure that there are no air bubbles in the syringe.
2
332 ÄKTA avant User Manual 29035184 AE
7 Maintenance
7.7 Calibration procedures
7.7.4 Calibrate the UV monitors
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