DescriptionInstruction name
Is used to fill the system with the selected BufferPro
buffer composition and stabilize the pH before the flow
is directed to the column. The wash flow can be directed
to either the waste position of the injection valve or the
outlet valve.
System wash BufferPro
Is used to set the start concentration of the quaternary
gradient. This instruction is often used together with the
instruction Quaternary Gradient. The sum of the start
concentrations of Q1 to Q4 must be 100%.
Quaternary start concen-
trations
Defines the length and end concentration of the quater-
nary gradient. The start values are set by the instruction
Quaternary start concentration. The sum of the target
concentrations Q1 to Q4 must be 100%.
Quaternary gradient
Is used to set or change the pH when automatic buffer
preparation is active during a method run.
BufferPro pH
Is used to set higher isocratic flow rates. This can be
useful in column packing.
In ÄKTA avant 25 both pump A and pump B generates
flows up to 50 ml/min.
In ÄKTA avant 150 both pump A and B generate flows
up to 300 ml/min.
Column packing flow
478 ÄKTA avant User Manual 29035184 AE
9 Reference information
9.8 Using Manual instructions
9.8.1 Manual instructions - Pumps and pressures
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