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GE AKTA avant - Page 67

GE AKTA avant
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Current mixing percentage for Q1-Q4 are shown in Process Picture in System Control.
The curves for Q1-Q4 can be shown in a chromatogram and stored in the result data
for documentation and for further studies in Evaluation.
Ports of Quaternary valve
The following illustration shows the ports of the Quaternary valve.
DescriptionPort
Buffer inletsQ1-Q4
Outlet to System pump A via Inlet valve AA
Outlet to System pump B via Inlet valve BB
BufferPro
The BufferPro tool allows automatic mixing of buffers during a run. Buffer preparation
can be performed using corresponding acid/base buffer systems. Buffer system, concen-
tration, pH and salt gradient are defined in the Method Editor.
In ÄKTA avant 25 BufferPro can be run at flow rates up to 25 ml/min. In ÄKTA
avant 150 BufferPro can be run at flow rates up to 40 ml/min.
Note:
When using BufferPro, immerse the Q inlets in the solutions according to the following
table.
SolutionInletSolutionInlet
WaterQ3Buffer stock solutionQ1
Gradient salt stock solutionQ4Acid or baseQ2
ÄKTA avant User Manual 29035184 AE 67
3 Standard instrument modules
3.4 Inlet valves

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