ActionStep
Enter the desired values for Control and Setpoint. Check the Deviation
Alarm and set the alarm limits if desired.
8
Click OK.9
Prepare the pH sensor
Follow the instructions below to prepare the pH sensor.
In dual mode, adjust the sensor calibration on each Cellbag bioreactor sepa-
rately.
Note:
ActionStep
When the medium is equilibrated to operating conditions, move the cursor
over the pH sensor icon in the Process Picture, and set Reading On in the
menu that appears.
1
Wait until the value is stable.
Click the right-hand side of the Sampling button to prepare for sampling.2
Result: The system will enter sampling mode.
Note:
The system will be in sampling mode as many minutes as set in Settings:Rock-
ing:Sampling:Pause and at an angle set in Settings:Rocking:Sampling:Stop
angle in the Process Picture.
Take a sample to verify that the pH value shown by the system matches the
pH measured with a calibrated reference instrument. If the deviation is
larger than approximately 0.5 pH units, make sure that no air bubble is
present.
3
For instructions on how to remove air bubbles, see troubleshooting in
ReadyToProcess WAVE 25 System Handbook.
Continue with the calibration adjustment only if the deviation is less than
0.5 pH units.
4
Select System:Calibrate in System Control.5
Select pH sensor in the Monitor to calibrate drop down menu.6
Enter the actual pH value in the Enter reference pH field.7
Click Calibrate.8
Close the Calibration dialog.9
142 ReadyToProcess WAVE 25 Operating Instructions 29009597 AD
5 Operation
5.3 Prepare for cultivation
5.3.4 Prepare the sensors
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