5.4.1 Inoculate the culture
Instructions
Follow the instructions below to inoculate the Cellbag bioreactor.
Make sure that the key culture parameters pH, DO and temperature are stable
before inoculation.
Note:
ActionStep
Make sure that the inlet tubing and the tubing connected to the inoculum
container are clamped.
1
Using sterile techniques, connect tubing from the inoculum container to the
inlet tubing, using e.g. tube fusing equipment or a ReadyMate™ connector.
2
Unclamp the inlet tubing and inoculum container tubing.3
Transfer the desired volume of inoculum into the bag using a pump or
gravity flow.
4
ReadyToProcess WAVE 25 Operating Instructions 29009597 AD 145
5 Operation
5.4 Perform cultivation
5.4.1 Inoculate the culture
To Next Page
Loading...
