GE ReadyToProcess WAVE 25 User Manual

5.4.1 Inoculate the culture
Instructions
Follow the instructions below to inoculate the Cellbag bioreactor.
Make sure that the key culture parameters pH, DO and temperature are stable
before inoculation.
Note:
ActionStep
Make sure that the inlet tubing and the tubing connected to the inoculum
container are clamped.
1
Using sterile techniques, connect tubing from the inoculum container to the
inlet tubing, using e.g. tube fusing equipment or a ReadyMate™ connector.
2
Unclamp the inlet tubing and inoculum container tubing.3
Transfer the desired volume of inoculum into the bag using a pump or
gravity flow.
4
ReadyToProcess WAVE 25 Operating Instructions 29009597 AD 145
5 Operation
5.4 Perform cultivation
5.4.1 Inoculate the culture

GE ReadyToProcess WAVE 25 Specifications

General

Brand	GE
Model	ReadyToProcess WAVE 25
Category	Laboratory Equipment
Language	English