5.3.3 Add and equilibrate culture medium
Tare the scale
Tare the scale with all the equipment on the tray, such as lid, Cellbag bioreactor, and
filter heater before starting a run. For optimal control, the measured weight should be
the same as the weight of the culture. The weight measurement is used as input for the
regulation of temperature, pH, and media control.
In dual mode, it is important for correct weight measurement to tare the scale, even if
the weight distribution appears to be even.
Load cells are temperature-sensitive. Keep the ambient temperature constant
to minimize effects on weight measurement.
Note:
Follow the instructions below to tare the scale.
ActionStep
Set the rocker stop angle to 0° and check that the tray is in a horizontal po-
sition.
1
Open Settings:Weight from the Process Picture in System Control.2
In dual mode, the weights of the two bioreactors are displayed separately.
Check that the weight distribution is even by reading the weight percentage
values for the rocker feet. The values should not differ more than ±5%, and
the optimal weight distribution is 25% on each load cell. Turn the adjustable
foot if necessary, see Adjustable foot, on page 46.
3
Make sure that the lid and all other equipment that will be used during the
run is placed on the tray, and that no tubing weighs down the tray.
4
Click Tare.5
138 ReadyToProcess WAVE 25 Operating Instructions 29009597 AD
5 Operation
5.3 Prepare for cultivation
5.3.3 Add and equilibrate culture medium
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