1035 Operation ..............................................................................................................
1045.1 Set up the system .................................................................................................................................
1055.1.1 Select the tray and Cellbag bioreactor .................................................................................
1065.1.2 Attach and detach tray ................................................................................................................
1095.1.3 Prepare pH and DO sensors ......................................................................................................
1125.1.4 Attach the Cellbag bioreactor ...................................................................................................
1145.1.5 Prepare the pump ..........................................................................................................................
1195.1.6 Connect gas to the system ........................................................................................................
1235.2 Start and configure the system .....................................................................................................
1245.2.1 Start the system and log on to UNICORN ...........................................................................
1255.2.2 Connect to the system .................................................................................................................
1275.2.3 Configure system properties .....................................................................................................
1295.2.4 Configure system settings ..........................................................................................................
1315.2.5 Start a run .........................................................................................................................................
1355.3 Prepare for cultivation ........................................................................................................................
1365.3.1 Inflate the Cellbag bioreactor ...................................................................................................
1375.3.2 Adjust pump parameters ...........................................................................................................
1385.3.3 Add and equilibrate culture medium .....................................................................................
1415.3.4 Prepare the sensors ......................................................................................................................
1445.4 Perform cultivation ..............................................................................................................................
1455.4.1 Inoculate the culture .....................................................................................................................
1465.4.2 Monitor the run ...............................................................................................................................
1485.4.3 End a run ...........................................................................................................................................
1506 Maintenance .........................................................................................................
1516.1 Calibration ................................................................................................................................................
1536.2 Cleaning ....................................................................................................................................................
1546.3 Cleaning before planned service ...................................................................................................
1557 Troubleshooting ...................................................................................................
1567.1 ReadyToProcess WAVE 25 system ................................................................................................
1577.2 ReadyToProcess WAVE 25 rocker .................................................................................................
1617.3 ReadyToProcess CBCU .......................................................................................................................
1627.4 ReadyToProcess Pump 25 ................................................................................................................
1637.5 UNICORN System Control .................................................................................................................
1658 Reference information ........................................................................................
1668.1 Chemical resistance ............................................................................................................................
1678.2 Health and Safety Declaration Form ...........................................................................................
169Index .......................................................................................................................
4 ReadyToProcess WAVE 25 Operating Instructions 29009597 AD
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