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4.2. Operating procedure with SUPRASPOR
SUPRASPOR’S BIOACID ACTIVITY
The balanced association of the hydrogen peroxide active principle with ethyl alcohol and non-ionic surfactant
makes SUPRASPOR a disinfectant product with a broad action spectrum, including negative gram bacteria
(Escherichia, Pseudomonas) and positive gram bacteria (Staphylococcus sp., methicilin resistant
Staphylococcus aureus - MRSA, Enterococci), yeasts (Candida), fungi, viruses (HIV, HBV, HCV),
mycobacteria and spores. Hydrogen peroxide is activated against a broad range of microorganisms, including
bacteria, yeasts, fungi, viruses and spores. Hydrogen peroxide accelerated at 0.5% has demonstrated
bactericidal and virucidal activity in 1 minute and mycobactericidal and fungicidal activity in 5 minutes.
Bactericidal effectiveness and stability of hydrogen peroxide in urines has been demonstrated against a variety
of pathogens associated with the healthcare area; organisms with a high catalysis activity (e.g. S. aureus, S.
marcescens and Proteus mirabilis) have required from 30 to 60 minutes of exposure to hydrogen peroxide at
0.6% for a reduction of the cellular count of 108, while organisms with a lower catalysis activity (e.g. E. coli,
Streptococci species and Pseudomonas species) have required only 15 minutes of exposure. In a study of
3%, 10% and 15% of HP, to reduce the bacterial population in a spacecraft, a complete killing of 106 spores
(e.g. Bacillus species) 10% of HP and 60 minutes of contact were necessary. A 3% concentration for 150
minutes killed 106 spores in 6 of the 7 studies made. A hydrogen peroxide solution at 10% resulted in a
decrease of 103 Bacillus atrophaeus spores, and a reduction > 105 when tested against 13 other pathogens
in 30 minutes at 20 °C.
A hydrogen peroxide solution at 3% was ineffective against VRE after 3 and 10 minutes of exposure, and only
caused a reduction of 2 logs of the number of Acanthamoeba cysts in approximately 2 hours. A stabilised
solution at 7% proved sporicidal after 6 hours of exposure, mycobactericidal (20 minutes), fungicidal (5
minutes) at complete concentration, virucidal (5 minutes) and bactericidal (3 minutes) diluted 1:16 when the
carrier test was used. The HP solution at 7% was tested after 14 days of stress (in the form of carriers loaded
with germs and respiratory therapy equipment) and proved sporicidal (> 7 log10 of reduction in 6 hours),
mycobactericidal (> 6.5 log10 of reduction in 25 minutes), fungicidal (> 5 log10 of reduction in 20 minutes),
bactericidal (> 6 log10 of reduction in 5 minutes) and virucidal (> 5 log10 of reduction in 5 minutes). Synergic
sporicidal effects were observed when the spores were exposed to a combination of HP (5.9% - 23.6%) and
peracetic acid. Other studies demonstrated the antiviral activity of HP against rhinoviruses. The time required
for the inactivation of 3 serotytpes or rhinoviruses using 3% of hydrogen peroxide was 6-8 minutes.
The bioacid activity tests, according to European standards in force (published by CEN/TC 216), were
conducted by a certified Test Facility operating according to GLP (Good Laboratory Practice), on the solution
as is. The following table shows references to the standards, the operating conditions (clean or dirty conditions)
and the results of these tests.
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