9.1 CALIBRATION PROCEDURE OF
PHOTOMETER 5010
SUBJECT: CUVETTE TEMPERATURE
RIELE BERLIN Photometer 5010 / V4 9.1.1 25.08.2003
1. Purpose
This calibration procedure describes the calibration of the cuvette temperature of Photometer 5010. The
meaning of calibration is to determine the difference between the true temperature and the set
temperature. The procedure is to carry out for the temperatures which are used by the customer.
2. General description and measuring theory
The temperature is measured with a selected thermistor-probe. The thermistor is supplied by RIELE. The
certified tolerance of this thermistor is ± 0.1 °C.
The photometer-cuvette temperature is measured in case of using the single cuvette adaptor in an
aluminium-dummy cuvette or in case of using the flow-through cuvette adaptor in the original flow-through
cuvette.
The resistance of the thermistor is measured by a 4½ digit digital multimeter (DMM). The requirements of
the DMM are given in chapter 7. The corresponding temperature is listed in chapter 8.
The implementation of the whole procedure lasts approximately 1.5 hours.
3. Operating conditions
The calibration should be performed under normal operating conditions, i.e.
• Room temperature: 15 °C to 30 °C
• Relative humidity: 40 % to 80 %
• No ventilation or draft
Note that the variation of the room temperature should not exceed ± 2 °C during the calibration process.
4. Traceability of the calibration standards
The traceability to the National Temperature Standard is given by selected thermistor and a calibrated
DMM. The last Calibration Certificate of both, the DMM and the thermistor, must not be older than
12 months. If the 24 month accuracy of the DMM fulfils the requirements in chapter 7, the last DMM
Calibration Certificate can be up to 24 months old.
5. Test procedure
a. In case of using the single cuvette adaptor insert the dummy-cuvette instead of a square cell.
In case of using the flow-through adaptor remove the joint inlet tube cuvette / aspirate tubing and
the outlet tube cuvette.
Pipette 200 µl distilled water in the dummy-cuvette or the flow-through cuvette.
b. Connect the thermistor lead to the two-wire resistance input of the DMM.
c. Insert the thermistor-probe into the hole of the photometer dummy-cuvette or the hole of the outlet
tube cuvette of the flow-through cuvette.
Caution: Handle the delicate probe with care! Pay attention to the correct position of the probe.
Avoid bubbles within the aperture of the flow-through cuvette.
d. Switch on the DMM and Photometer 5010 and wait 15 minutes for warming up.
e. Select the two-wire resistance function and appropriate range on the DMM. The resistance of the
thermistor is in the range of 6 kOhm up to 10 kOhm for the temperature range of 25 °C up to
37 °C. The table of resistance of thermistor ID 090-063 shows the assigned resistance for a given
temperature.
f. Enter the service program level.
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