Cleaning the extraction cone 5-35
• Ultrasonic bath.
• Source of oil-free, inert gas (for example, nitrogen) for drying (air-drying
optional).
• Wash bottle containing HPLC-grade (or better) 1:1 methanol/water.
• Large beaker.
To clean the extraction cone
1. Immerse the extraction cone in the glass vessel containing 1:1
methanol/water.
Tip: If the extraction cone is obviously contaminated, use 45:45:10
methanol/water/formic acid.
2. Place the vessel in the ultrasonic bath for 30 minutes.
Requirement: If you used formic acid in the cleaning solution, do as
follows:
• Rinse the extraction cone by immersing it in a glass vessel
containing water and then placing the vessel in the ultrasonic bath
for 20 minutes.
• Remove any residual water from the extraction cone by immersing
it in a glass vessel containing methanol and then placing the vessel
in the ultrasonic bath for 10 minutes.
Warning: The extraction cone can be contaminated with
biohazardous and/or toxic materials. Always wear
chemical-resistant, powder-free gloves while performing this
procedure.
Warning: Formic acid is extremely corrosive and toxic. Work with
extreme care, use a fume hood and suitable protective equipment.
Caution: The extraction cone is fragile. Never place it on its tip; always
place it on its flanged base.
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