3-8 Configuring the LockSpray Source
Installing the ESI small bore capillary option
The ESI small bore capillary option is for use with 1-mm UPLC columns
running at flowrates of 100 to 200 µL/min. The materials needed for this task
are in the Small-Bore Capillary kit.
Required materials
• Chemical-resistant, powder-free gloves
• Combined 2.5-mm Allen wrench and cone extraction tool
•10-mm wrench
•8-mm wrench
• 2 × 7-mm wrenches
•LC pump
• HPLC-grade (or better) 1:1 acetonitrile:water
• Sharp knife or PEEK tubing cutter
• From the Small-Bore Capillary kit:
– Capillary
– Small-bore, UNF coupler (slide port)
– Collar nut (thumb nut)
– PTFE liner tube
– Conductive sleeve
– 2 × 1/16-inch ferrules
• Metal gasket for the probe tip
• Safety goggles
Caution: To avoid damage from excessive pressure, do not exceed flow
rates of 200 µL/min through the ESI probe when using the small-bore
capillary.
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