Overview of the NanoLockSpray source 4-3
Schematic of the NanoLockSpray source
Spray indexing permits acquiring sample and lock-spray data in separate
data channels, and the baffle design ensures negligible cross-talk between the
two sprays. The lock-spray data are used to calculate a correction factor for
the mass-scale calibration, which is then applied to the sample data, providing
exact-mass information.
Sample sprayer
You can use the NanoLockSpray source with different NanoFlow sprayers.
For instructions on how to set up these sprayers, see page 4-4.
LockSpray sprayer
The LockSpray sprayers for the LockSpray source and NanoLockSpray source
operate as part of the instrument's IntelliStart Fluidics system. Fitted with a
500-µL pump, the LockSpray sprayer operates at 0.5 µL/min. You must choose
the concentration of the lock-spray reference solution that gives a suitable ion
intensity.
Sample sprayer
Sample cone
LockSpray sprayer
Baffle
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