5 First Operating Steps | 5.4 Acquiring an Image ZEISS
2. Alternatively, to activate the chamberscope, click the TV icon in the Toolbar or press the
Camera button on the control panel.
à
The inside of the specimen chamber is visible
in the Image Area.
3. In the SEM Controls panel, select the Stage tab.
4. Activate the Track Z checkbox.
à The current working distance (WD) is displayed in the Data Zone.
INFO: If the Data Zone is disabled, enable it via Menu Bar > View > Data Zone >
Show Data Zone.
5. Use the dual joystick to drive the specimen stage downwards to a low position.
Alternatively, use the soft joystick via Tools > Goto Panel > Soft Joystick.
NOTICEObserve the stage movement via camera to avoid crashing.
5.4.2.3 Venting the Specimen Chamber
WARNING
Suffocation hazard due to lack of oxygen
Gaseous dry nitrogen is used to vent the specimen chamber during specimen exchange. Inhal-
ing nitrogen may cause unconsciousness.
4 During specimen exchange, keep the chamber door open as short as possible.
4 Do not inhale the air from within the specimen chamber.
4 Ensure that the area around the microscope is sufficiently ventilated.
4 If you begin to experience symptoms of asphyxia (for example: rapid breathing, loss of
mental alertness and/or muscular coordination, depression of sensations, emotional insta-
bility, fatigue) leave the room immediately and inform the facility’s safety officer.
Procedure
1. In the SEM Controls panel, select the Vacuum tab.
2. Click Vent.
à The Vent message box is displayed.
3. To start venting, click Yes.
INFO: If the Stage is not initialized system message is displayed, refer to Initializing the
Stage [
}
115].
à The specimen chamber is purged with gaseous nitrogen.
64 Instruction Manual ZEISS EVO | en-US | Rev. 10 | 354706-0780-006
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