5 First Operating Steps | 5.4 Acquiring an Image ZEISS
5.4.9.8 Visualizing Details on the Specimen Surface
Procedure
1. Select a detail on the specimen surface.
2. Verify the Magnification/Focus function is activated.
3. To adjust the magnification, hold down the left mouse button and drag the mouse within
the Image Area in left/right direction.
à The current magnification is indicated in the Status Bar.
4. To adjust the focus, change the working distance. Hold down the mouse wheel and drag
the mouse within the Image Area in left/right direction.
à Alternatively, you can press <Ctrl+F> to use the fine autofocus or you can press <Ctrl
+Shift+F> to use the coarse autofocus.
à The current working distance is indicated in the Status Bar.
5. Adjust contrast and brightness again.
5.4.10 Optimizing the Image
Once you have generated an initial image, you can adjust various parameters to optimize the im-
age.
Info
The following procedure describes how to manually improve the image quality. You can also
use the Auto Aperture Alignment Function.
Info
The following procedure describes the best way to quickly optimize the image without the
control panel. You can also use the control panel to adjust aperture alignment, magnification/
focus and brightness/contrast.
This procedure consists of the following steps:
1. Adjusting the Magnification [
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2. Limiting the Scan Field [
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3. Aligning the Aperture [
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4. Selecting the Scan Speed [
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5. Correcting Astigmatism [
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6. Adjusting Final Magnification, Contrast and Brightness [
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5.4.10.1 Adjusting the Magnification
Procedure
1. Step by step, raise the magnification to about twice the desired final magnification (e.g.
50,000 x) and focus in between.
Use the Coarse mode or the Fine mode of adjustment, as appropriate. To toggle between
Fine and Coarse mode, in the Status Bar, click
or .
2. To adjust the magnification and the focus, hold down the left mouse button or the mouse
wheel, respectively, and drag the mouse within the Image Area.
76 Instruction Manual ZEISS EVO | en-US | Rev. 10 | 354706-0780-006
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