NOTES
• To ensure proper liquid level detection when testing liquids with
extremely low dielectric properties, such as de-ionized water, use a
primary tube filled to near capacity with the liquid.
• To ensure proper liquid level detection when testing samples con-
tained in ampules, use a lint-free cloth or swab to carefully remove
any liquid film present at the ampule opening.
Particulate matter and certain bacteria can also cause premature crystal-
lization. In blood, centrifugation normally eliminates such contaminants.
Centrifugation or filtration through an inert filter may remove particulate
matter in urine.
Verifying calibration daily
Like most measuring instruments, osmometers must be calibrated using
standards. Your A
2
O was calibrated at the factory. The probe and cali-
bration parameters are stored in non-volatile memory. Thus, when
“Ready” is displayed, your A
2
O osmometer is calibrated and ready to
run. However, the calibration should always be verified during operation
to be sure that none of the parameters affecting calibration have changed.
(If recalibration is required, see Chapter 4).
NOTE To verify the accuracy of your instrument, test products with
known values within your reportable range (i.e., Clinitrol™ 290
mOsm/kg, Protinol
®
Protein-based Controls, Renol™ Urine
Osmolality Controls).
Sample test procedure
Following is a recommended procedure for testing a sample of unknown
osmolality.
1. Select a reference solution or NaCl standard with a freezing point
close to that expected for your unknown sample (Advanced
Clinitrol™ 290 Reference Solution is recommended for serum). Swirl
and carefully open an ampule of the selected solution. Place the
ampule into the primary carousel using one of the adapters provided.
Instrument Operation
33
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