1290 Infinity Quaternary Pump User Manual 75
Using the Module
4
Purging the Pump
Purging the Pump
When the solvents have been exchanged or the pumping system has been
turned off for a certain time (for example, overnight) oxygen will re-diffuse
into the solvent channel between the solvent reservoir, vacuum degassing unit
(when available in the system) and the pump. Solvents containing volatile
ingredients will slightly lose these. Therefore purging of the pumping system is
required before starting an application.
1 Initiate a purge in the controlling software with a Purge flow set to
3 – 5 ml/min per channel.
2 Flush all tubes with at least 30 ml of solvent.
Tab le 6 Choice of Priming Solvents for Different Purposes
Activity Solvent Comments
After an installation
When switching between reverse phase and
normal phase (both times)
Isopropanol
Isopropanol
Best solvent to flush air out of the system
Isopropanol is miscible with both normal phase
and reverse phase solvents.
After an installation Ethanol or Methanol Alternative to Isopropanol (second choice) if no
Isopropanol is available
To clean the system when using buffers
After a solvent change
Bidistilled water
Bidistilled water
Best solvent to re-dissolve buffer crystals
Best solvent to re-dissolve buffer crystals
Before turning off system for an extended
period of time
Organic or 10 %
isopropanol in water
The pump should never be used for priming/purging empty tubings (never let the pump
run dry). Use a syringe to draw enough solvent for completely filling the tubings to the
pump inlet before continuing to prime with the pump.
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