Applied Biosystems 7300 User Manual

Chapter 6 Performing the Pure Dye Calibration
Preparing Plates and Performing the Pure Dye Calibration
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide 75
Notes
6
While the SDS Software performs the pure dye
calibration, it locks the controls of the Pure Spectra
Calibration Manager.
Unloading the Plate
PHYSICAL INJURY
HAZARD. During instrument operation, the sample
block can be heated to 100 °C. Before performing the
following procedure, be sure to wait until the sample
block reaches room temperature.
1. When the SDS software completes the run:
a. Press the tray to open it.
b. Remove the pure dye plate from the tray.
c. Press the tray to move it into the instrument.
d. Place the pure dye plate inside its packaging
sleeve, and return it to the spectral
calibration kit in the freezer.
Note: Do not discard the pure dye plates. If
you store the plates in their packaging
sleeves at –20 to –25 ° C, you can use the
pure dye plates up to three times after you
open them.
Calibrating Remaining Dyes
1. Click .
2. Repeat the procedures in “Preparing and Loading
a Pure Dye Plate” on page 73 through
“Calibrating Remaining Dyes” on page 75 to run
the remaining pure dye plates (JOE, NED, ROX,
SYBR Green, TAMRA, VIC).
Note: If you are using a 7500 or 7500 Fast
instrument, also perform calibrations for the CY3,
CY5, and TEXAS RED pure dyes .
a b
c
d
Click

Applied Biosystems 7300 Specifications

General

Brand	Applied Biosystems
Model	7300
Category	Laboratory Equipment
Language	English