Chapter 7 Verifying the Instrument Performance
Analyzing the TaqMan
®
RNase P Plate Data
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide 95
Notes
7
5. Display the data. In the Amplification Plot tab:
a. Click the upper-left corner of the plate grid
to select all wells.
b. Select DataCt vs. Well Position to
display the Ct vs. Well Position plot.
6. Verify the uniformity of each replicate
population by comparing the groupings of C
T
values.
Note: Experimental error may cause some wells
to be amplified insufficiently or not at all. These
wells typically produce C
T
values that differ
significantly from the average for the associated
replicate wells. If included in the calculations,
these outlying data (outliers) can result in
erroneous measurements.
7. If outliers are present, reanalyze the run:
a. In the plate grid, select the wells that
produced the outlying data.
b. Select ViewWell Inspector.
Click
Outliers
1
13
25
37
49
61
73
85
2
14
26
38
50
62
74
86
3
15
27
39
51
63
75
87
4
16
28
40
52
64
76
88
5
17
29
41
53
65
77
89
6
18
30
42
54
66
78
90
7
19
31
43
55
67
79
91
8
20
32
44
56
68
80
92
9
21
33
45
57
69
81
93
10
22
34
46
58
70
82
94
11
23
35
47
59
71
83
95
12
24
36
48
60
72
84
96
The numbers on the X-axis of the plot correspond to the
wells of the 96-well plate. Beginning at well A1, the wells
are numbered from left-to-right, and top-to-bottom.
Outliers
7300/7500 system plate
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