Chapter 7 Verifying the Instrument Performance
Analyzing the TaqMan
®
RNase P Plate Data
96 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Notes
c. In the Well Inspector, select Omit Well.
d.
Click (or select
Analysis
Analyze
)
to
reanalyze the run without the outlying data.
8. Repeat step 7 on the previous page for other
wells with outlying data.
IMPORTANT! Up to six wells from each
replicate group in a RNase P plate can be omitted
to meet specifications. If the analysis contains
more than six outliers,
troubleshoot the RNase P
verification run. See “Troubleshooting” on
page 99.
9. In the Results tab, select the Standard Curve
tab.
10. In the Standard Curve tab, verify that the R2
value is ≥0.990.
11. In the Results tab, select the Report tab.
Select
Omitted well
is crossed-out
Select
Must be ≥0.99
Select
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